Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection

doi: 10.1016/j.jcmgh.2019.03.003

Figure Lengend Snippet: Antibodies Used in Immunofluorescence Images and FACS Analysis

Article Snippet: TFF2 , Bioss Antibodies , bs-1921R , Unconjugate.

Techniques: Immunofluorescence

Journal: Cancer Prevention Research

Article Title: Goblet Cell Ratio in Combination with Differentiation and Stem Cell Markers in Barrett Esophagus Allow Distinction of Patients with and without Esophageal Adenocarcinoma

doi: 10.1158/1940-6207.capr-16-0117

Figure Lengend Snippet: Figure 2. A, TFF2 IHC of Barrett esophagus metaplasia in the pL2-IL1b mouse model with increasing status of early (2 months) to metaplastic (5 and 10 months) Barrett esophagus and dysplasia (12 months). B, Histopathologic score in correlation with TFF2 staining in the pL2-IL1b mouse model (ANOVA: P < 0.0001, R2: 0.8728, F: 54.91). C, TFF2 staining in human biopsies from Barrett esophagus or adjacent to EAC tissue was scored from 0 to 4, percentage of each score in both groups is shown (4%/9%/22%/35%/31% in Barrett esophagus as well as 10%/62%/22%/4%/2% in EAC). D, Representative TFF2 staining of human biopsies from Barrett esophagus or adjacent to EAC tissue.

Article Snippet: For immunohistochemical (IHC) staining, incubation was performed using primary antibodies: DCLK1 (Abgent Inc., 1:200), TFF2 (Proteintech Group, 1:1,000), and LGR5 (Abcam, 1:100).

Techniques: Staining

Journal: Cancer Prevention Research

Article Title: Goblet Cell Ratio in Combination with Differentiation and Stem Cell Markers in Barrett Esophagus Allow Distinction of Patients with and without Esophageal Adenocarcinoma

doi: 10.1158/1940-6207.capr-16-0117

Figure Lengend Snippet: Figure 5. Logistic regression models for all 4 biomarkers. The ROC plots indicate that TFF2 shows the best discrimination between Barrett esophagus and EAC, with an AUC of 87.2%, followed by DCLK1 (AUC 83.4%), goblet cell ratio (AUC 79.4%), and LGR5 (AUC 71.4%).

Article Snippet: For immunohistochemical (IHC) staining, incubation was performed using primary antibodies: DCLK1 (Abgent Inc., 1:200), TFF2 (Proteintech Group, 1:1,000), and LGR5 (Abcam, 1:100).

Techniques:

Journal: Digestive diseases and sciences

Article Title: Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.

doi: 10.1007/s10620-011-1724-9

Figure Lengend Snippet: Fig. 2 Heterogeneity of the trefoil factor family 2 molecule in gastric juice, and its influence on the present assay method. a Recombinant trefoil factor family 2 (TFF2) (lane Ag) and patients’ gastric juices (lanes 1–4), treated with Laemmli buffer (without 2-mercap- toethanol), were loaded onto 4–20% gradient polyacrylamide gels. After electrophoresis, separated proteins were transferred electropho- retically onto a nitrocellulose membrane. The bands containing TFF2 were visualized by immunoblot analysis using anti-TFF2 antibody. b To investigate the effect of heterogeneity of the TFF2 molecule, we measured TFF2 levels in the serial dilutions of mixed-gastric juice samples that were prepared using two gastric juices (lanes 2 and 4 in a). Data are expressed as mean ± standard deviation (SD) derived from triplicate determinations in each of the two separate experiments

Article Snippet: Briefly, commercially available polystyrene immunoplates (Nunc, Roskilde, Denmark) were coated with goat polyclonal anti-human TFF2 antibody (2,000-fold dilution) (R&D systems, Minneapolis, MN) in 0.1 mol/l sodium bicarbonate, pH9.6 (1.0 mg protein/l) for 24 h at 4 C. Plates were washed four times with PBS-Tween after each of the subsequent incubation steps.

Techniques: Recombinant, Electrophoresis, Membrane, Western Blot, Standard Deviation, Derivative Assay

Journal: Digestive diseases and sciences

Article Title: Pathophysiological investigation of the gastric surface mucous gel layer of patients with Helicobacter pylori infection by using immunoassays for trefoil factor family 2 and gastric gland mucous cell-type mucin in gastric juice.

doi: 10.1007/s10620-011-1724-9

Figure Lengend Snippet: Fig. 3 TFF2 concentration in gastric juices from healthy individuals and patients. a TFF2 concentration in gastric juices from patients with gastritis, gastric ulcer (GU), duodenal ulcer (DU) and healthy individuals (control). b TFF2 concentration in gastric juices from patients with H. pylori infection (HP (?)) and in H. pylori-negative patients (HP (-)). c TFF2 concentration in gastric juices from patients before and after the H. pylori eradication. *p \ 0.05; **p \ 0.01; NS not significant

Article Snippet: Briefly, commercially available polystyrene immunoplates (Nunc, Roskilde, Denmark) were coated with goat polyclonal anti-human TFF2 antibody (2,000-fold dilution) (R&D systems, Minneapolis, MN) in 0.1 mol/l sodium bicarbonate, pH9.6 (1.0 mg protein/l) for 24 h at 4 C. Plates were washed four times with PBS-Tween after each of the subsequent incubation steps.

Techniques: Concentration Assay, Control, Infection

Journal: JCI Insight

Article Title: Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing

doi: 10.1172/jci.insight.152616

Figure Lengend Snippet: ( A ) The t-SNE plot that showed the distribution of cancer cell lineages (yellow, n = 2196 cells) within the atlas. Cancer cell populations were reclustered into 9 subclusters (color coding). ( B ) Top 5 marker genes of 15 major cell types identified in this profile. ( C ) Annotation by left-sided and right-sided CRC cells. ( D ) The fraction of cells that originated from left-sided and right-sided CRC samples for 9 subgroups identified in this profile. ( E ) Kaplan-Meier survival curves of OS based on HSPA1A, GOLGB1, AGR3, MUC5AC, NTS, SPINK4, CLCA1, OLFM4, and PIGR expression using the online bioinformatics tool Kaplan-Meier Plotter. ( F ) Violin plots and immunochemistry display the distribution of expression of AGR2, AGR3, MUC5AC, SPINK4, TFF1, and TFF2 between left-sided and right-sided CRC. Scale bars: 100 µm.

Article Snippet: Anti-TFF1 antibody (13734-1-AP) and anti-TFF2 antibody (13681-1-AP) were purchased from Proteintech.

Techniques: Marker, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Presence and localization of TFFs in synovial membrane (SM). ( A ) Semi-quantitative PCR analysis of TFFs mRNA in human SM of five healthy (28, 48, 54, 78, and 92 years (Lines 1, 2, 3, 4, and 5)), rheumatoid arthritis (RA) (Lines 6 and 7) and osteoarthritis (OA) (Lines 8 and 9) samples. Line 10 represents the negative control (without cDNA template), and human stomach serves as positive control (Line 11). Beta-actin (β-actin) was used as the loading control. ( B ) Immunohistochemical analysis of TFF1, -2 and -3 in human SM of 22 ( a , b , c ), 48 ( d , e , f ), and 83 ( g , h , i ) year-old healthy donors. TFF3 is present in each examined sample ( c , f , i ). TFF1 ( a , d , g ) and TFF2 ( b , e , h ) reveal negative results irrespective of the donor’s age. Insets show magnifications. Scale bars: 100 μm. Red staining indicates positive antibody reaction.

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Real-time Polymerase Chain Reaction, Negative Control, Positive Control, Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Detection and quantification of TFF peptides in synovial fluid (SF). ( A ) Western blot analysis of TFFs in human SF of healthy (Line 1 and 2), osteoarthritis (OA; Line 3 and 4), and rheumatoid arthritis (RA; Line 5 and 6) samples. Proteins of the human stomach serving as positive control (Line 7) were included in the test. Actin (≈ 43 kDa) and alpha-1-antitrypsin (≈ 51 kDa) were used as loading control. Molecular weight marker is shown on the right. ELISAs of TFF1 ( B ), –2 ( C ), and –3 ( D ) in human SF of healthy ( n = 13), OA ( n = 20) and RA ( n = 20) samples. Mean values are: TFF1: 50.87 pg/mg (healthy), 31.16 pg/mg (OA), and 33.04 pg/mg (RA); TFF2: 13.69 pg/mg (healthy), 8.97 pg/mg (OA), 203.50 pg/mg (RA); TFF3: 7807.29 pg/mg (healthy), 2833.90 pg/mg (OA), 4083.92 pg/mg (RA). The protein concentration is expressed in pg/mg and visualized as mean value and standard error of the mean (SEM). * indicates significant differences performing the Mann–Whitney U -Test (significance level p ≤ 0.05).

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Western Blot, Positive Control, Molecular Weight, Marker, Protein Concentration, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: ELISA quantification of TFF2 in synovial fluid.

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Primers used for semi-quantitative PCR and real-time PCR.

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

doi: 10.3390/ijms20236105

Figure Lengend Snippet: Primary and secondary antibodies used for western blot (WB) and immunohistochemistry (IHC)

Article Snippet: mouse anti-TFF2 , WB , monoclonal , H00007032-M01, Abnova.

Techniques: Western Blot, Immunohistochemistry, Plasmid Preparation